Human Interleukin-6 Receptor Subunit Alpha, IL6R ELISA Kit

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

Concentration | O.D. | Average | Corrected ------------------------------------------ 3000 pg/ml | 2.845 | 2.873 | 2.763 2.901 1500 pg/ml | 1.433 | 1.457 | 1.347 1.481 750 pg/ml | 0.739 | 0.747 | 0.637 0.754 375 pg/ml | 0.397 | 0.376 | 0.266 0.355 187.5 pg/ml | 0.232 | 0.231 | 0.121 0.229 93.75 pg/ml | 0.16 | 0.157 | 0.047 0.153 46.875 pg/ml | 0.125 | 0.124 | 0.014 0.122 0 pg/ml | 0.104 | 0.11 | 0 0.116

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 405 | 17.2 | 4.2 Intra-assay | 2 | 20 | 943 | 32 | 3.4 Intra-assay | 3 | 20 | 2143 | 102 | 4.7 Inter-assay | 1 | 20 | 403 | 40.3 | 10 Inter-assay | 2 | 20 | 946 | 64.9 | 6.9 Inter-assay | 3 | 20 | 2147 | 61.8 | 2.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail