Influenza B Nucleoprotein ELISA Kit

Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs) : a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.328 | 2.409 | 2.308 2.491 1250 pg/ml | 1.348 | 1.32 | 1.219 1.293 625 pg/ml | 0.684 | 0.701 | 0.6 0.719 312.5 pg/ml | 0.407 | 0.404 | 0.303 0.402 156.25 pg/ml | 0.232 | 0.243 | 0.142 0.255 78.125 pg/ml | 0.149 | 0.158 | 0.057 0.167 39.0625 pg/ml | 0.131 | 0.128 | 0.027 0.126 0 pg/ml | 0.105 | 0.101 | 0 0.098

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 201 | 6.1 | 3 Intra-assay | 2 | 20 | 694 | 29.1 | 4.2 Intra-assay | 3 | 20 | 1781 | 60.1 | 3.4 Inter-assay | 1 | 20 | 200 | 18.3 | 9.2 Inter-assay | 2 | 20 | 698 | 45.3 | 6.5 Inter-assay | 3 | 20 | 1792 | 150 | 8.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail