Human Cartilage Oligomeric Matrix Protein, COMP ELISA Kit

Cartilage Oligomeric Matrix Protein (COMP), also referred to as Thrombospondin-5, is a non-collagenous extracellular matrix (ECM) protein and belongs to the subgroup B of the thrombospondin protein family. This protein is expressed primarily in cartilage, ligament, and tendon, and binds to other ECM proteins such as collagen I, II and IX with high affinities depending on the divalent cations Zn2+ or Ni2+. COMP is a secreted glycoprotein that is important for growth plate organization and function. It is suggested to play a role in cell growth and development, and recent studies have revealed the possible mechanism that it protects cells against death by elevating members of the IAP (inhibitor of apoptosis protein) family of survival proteins. Mutations in COMP cause two skeletal dysplasias, pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (EDM1), and up-regulated expression of COMP are observed in rheumatoid arthritis and certain carcinomas.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.64 | 2.664 | 2.537 2.689 1250 pg/ml | 1.592 | 1.566 | 1.439 1.54 625 pg/ml | 0.912 | 0.896 | 0.769 0.88 312.5 pg/ml | 0.503 | 0.498 | 0.3705 0.492 156.25 pg/ml | 0.294 | 0.291 | 0.164 0.288 78.125 pg/ml | 0.209 | 0.199 | 0.072 0.19 39.0625 pg/ml | 0.154 | 0.153 | 0.026 0.152 0 pg/ml | 0.133 | 0.127 | 0 0.121

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 281 | 22.7 | 8.1 Intra-assay | 2 | 20 | 758 | 39.2 | 5.2 Intra-assay | 3 | 20 | 1764 | 92.8 | 5.3 Inter-assay | 1 | 20 | 278 | 19.9 | 7.1 Inter-assay | 2 | 20 | 754 | 47.3 | 6.3 Inter-assay | 3 | 20 | 1756 | 85.6 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail