Human Kallikrein-13, KLK13 ELISA Kit

Tissue kallikrein 13 (hK13), also known as KLK-L4 (kallikrein-like gene 4), is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers and is regarded as a novel cancer biomarker. Besides, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 1.836 | 1.795 | 1.686 1.755 1250 pg/ml | 1.061 | 1.043 | 0.934 1.026 625 pg/ml | 0.647 | 0.673 | 0.564 0.7 312.5 pg/ml | 0.401 | 0.386 | 0.277 0.372 156.25 pg/ml | 0.224 | 0.237 | 0.128 0.251 78.125 pg/ml | 0.163 | 0.163 | 0.054 0.164 39.0625 pg/ml | 0.131 | 0.14 | 0.031 0.15 0 pg/ml | 0.103 | 0.109 | 0 0.116

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 450 | 38.6 | 8.6 Intra-assay | 2 | 20 | 938 | 43.9 | 4.7 Intra-assay | 3 | 20 | 1997 | 99.9 | 5 Inter-assay | 1 | 20 | 448 | 39.5 | 8.8 Inter-assay | 2 | 20 | 929 | 56.3 | 6.1 Inter-assay | 3 | 20 | 1979 | 134 | 6.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail