Human Extracellular Matrix Protein 1, ECM1 ELISA Kit

Extracellular matrix protein 1 (ECM1) is a secreted glycoprotein and playing a pivotal role in endochondral bone formation, angiogenesis, and tumour biology. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. Although ECM1 is not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. It also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.361 | 2.33 | 2.217 2.299 1250 pg/ml | 1.283 | 1.309 | 1.196 1.334 625 pg/ml | 0.752 | 0.715 | 0.602 0.677 312.5 pg/ml | 0.437 | 0.424 | 0.311 0.41 156.25 pg/ml | 0.247 | 0.261 | 0.148 0.274 78.125 pg/ml | 0.19 | 0.187 | 0.074 0.184 39.0625 pg/ml | 0.153 | 0.15 | 0.037 0.146 0 pg/ml | 0.12 | 0.113 | 0 0.106

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 317 | 23 | 7.2 Intra-assay | 2 | 20 | 774 | 53.8 | 6.9 Intra-assay | 3 | 20 | 1799 | 81.2 | 4.5 Inter-assay | 1 | 20 | 319 | 32.2 | 10.1 Inter-assay | 2 | 20 | 765 | 72.8 | 9.5 Inter-assay | 3 | 20 | 1817 | 139 | 7.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail