Human Insulin-Like Growth Factor-Binding Protein 3, IGFBP3 ELISA Kit

The Insulin-like Growth Factor (IGF) signaling system plays a central role in cellular growth, differentiation, and proliferation. IGFBP3 is the most abundant IGF binding protein in human serum and is a growth inhibitory, apoptosis-inducing molecule, capable of acting via IGF-dependent and IGF-independent mechanisms. It appears to function both by cell cycle blockade and the induction of apoptosis. IGFBP3 can be transported to the nucleus by an importin beta mediated mechanism, where it has been shown to interact with the retinoid X receptor alpha and possibly other nuclear elements. IGFBP3 antiproliferative signaling appears to require an active transforming growth factor-beta (TGF-beta) signaling pathway, and IGFBP3 stimulates phosphorylation of the TGF-beta signaling intermediates Smad2 and Smad3. IGFBP3 has IGF-independent roles in inhibiting cell proliferation in cancer cell lines. Nuclear transcription factor, retinoid X receptor (RXR) -alpha, and IGFBP3 functionally interact to reduce prostate tumor growth and prostate-specific antigen in vivo. Several clinical studies have proposed that individuals with IGFBP3 levels in the upper range of normal may have a decreased risk for certain common cancers. This includes evidence of a protective effect against breast cancer, prostate cancer, colorectal cancer, and lung cancer. Moreover, IGFBP3 inhibits insulin-stimulated glucose uptake into adipocytes independent of IGF.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.922 | 2.833 | 2.73 2.744 1250 pg/ml | 1.462 | 1.465 | 1.362 1.468 625 pg/ml | 0.736 | 0.77 | 0.667 0.804 312.5 pg/ml | 0.439 | 0.44 | 0.337 0.44 156.25 pg/ml | 0.251 | 0.268 | 0.165 0.285 78.125 pg/ml | 0.189 | 0.185 | 0.082 0.181 39.0625 pg/ml | 0.15 | 0.151 | 0.048 0.151 0 pg/ml | 0.106 | 0.103 | 0 0.1

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 293 | 10.8 | 3.7 Intra-assay | 2 | 20 | 650 | 16.7 | 2.6 Intra-assay | 3 | 20 | 1806 | 46.4 | 2.6 Inter-assay | 1 | 20 | 291 | 22.7 | 7.8 Inter-assay | 2 | 20 | 647 | 44.4 | 6.9 Inter-assay | 3 | 20 | 1808 | 133 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail