Human Tumor Necrosis Factor Receptor Superfamily Member 19L, RELT ELISA Kit

Receptor expressed in lymphoid tissues (RELT), also known as tumor necrosis factor receptor superfamily, member 19-like (TNFRSF19L), is a member of the TNF-receptor superfamily. This receptor is especially abundant in hematologic tissues. It has been shown to activate the NF-kappaB pathway and selectively bind TNF receptor-associated factor 1. RELT/TNFRSF19L is capable of stimulating T-cell proliferation in the presence of CD3 signaling, which suggests its regulatory role in immune response. RELT/TNFRSF19L is a type I transmembrane glycoprotein with a cysteine-rich extracellular domain, possessing significant homology to other members of the TNFR superfamily, especially TNFRSF19, DR3, OX40, and LTbeta receptor. RELT/TNFRSF19L is able to activate the NF-kappaB pathway and selectively binds tumor necrosis factor receptor-associated factor 1. RELT/TNFRSF19L is able to activate the NF-κB pathway and selectively binds tumor necrosis factor receptor-associated factor 1. Although the soluble form of RELT fusion protein does not inhibit the one-way mixed lymphocyte reaction, immobilized RELT/TNFRSF19L is capable of costimulating T-cell proliferation in the presence of CD3 signaling.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.457 | 2.401 | 2.267 2.345 1250 pg/ml | 1.263 | 1.275 | 1.141 1.287 625 pg/ml | 0.67 | 0.716 | 0.582 0.761 312.5 pg/ml | 0.403 | 0.414 | 0.28 0.425 156.25 pg/ml | 0.261 | 0.273 | 0.139 0.284 78.125 pg/ml | 0.209 | 0.2 | 0.066 0.191 39.0625 pg/ml | 0.17 | 0.166 | 0.032 0.161 0 pg/ml | 0.14 | 0.134 | 0 0.128

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 250 | 15.1 | 6 Intra-assay | 2 | 20 | 543 | 24.4 | 4.5 Intra-assay | 3 | 20 | 1429 | 62.4 | 4.4 Inter-assay | 1 | 20 | 250 | 24.8 | 9.9 Inter-assay | 2 | 20 | 546 | 39.7 | 7.3 Inter-assay | 3 | 20 | 1412 | 85.9 | 6.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail