Human Asialoglycoprotein Receptor 1, ASGR1 ELISA Kit

The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR) . ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1 (MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2) . ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.48 | 2.523 | 2.438 2.566 1250 pg/ml | 1.373 | 1.418 | 1.333 1.462 625 pg/ml | 0.729 | 0.748 | 0.663 0.767 312.5 pg/ml | 0.418 | 0.425 | 0.34 0.431 156.25 pg/ml | 0.233 | 0.244 | 0.159 0.255 78.125 pg/ml | 0.154 | 0.16 | 0.075 0.166 39.0625 pg/ml | 0.115 | 0.121 | 0.036 0.126 0 pg/ml | 0.086 | 0.085 | 0 0.084

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 256 | 10.8 | 4.2 Intra-assay | 2 | 20 | 801 | 29.7 | 3.7 Intra-assay | 3 | 20 | 1864 | 69.8 | 3.7 Inter-assay | 1 | 20 | 255 | 33.2 | 13 Inter-assay | 2 | 20 | 804 | 77.7 | 9.7 Inter-assay | 3 | 20 | 1886 | 163 | 8.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail