Human CD38 ELISA Kit

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 38 (CD38), also known as ADP-ribosyl cyclase, is a glycoprotein found on the surface of many immune cells (white blood cells), including CD4+, CD8+, B and natural killer cells. It shares several characteristics with ADP-ribosyl cyclase 2 CD157. CD38 is a multifunctional ectoenzyme that catalyzes the synthesis and hydrolysis of cyclic ADP-ribose (cADPR) from NAD+ to ADP-ribose. It also functions in cell adhesion, signal transduction and calcium signaling. CD38 has been used as a prognostic marker in leukemia. It can also be used to identify plasma cells.

Concentration | O.D. | Average | Corrected ------------------------------------------ 6000 pg/ml | 2.465 | 2.579 | 2.475 2.694 3000 pg/ml | 1.499 | 1.45 | 1.346 1.402 1500 pg/ml | 0.79 | 0.814 | 0.71 0.838 750 pg/ml | 0.499 | 0.483 | 0.379 0.467 375 pg/ml | 0.315 | 0.303 | 0.199 0.292 187.5 pg/ml | 0.193 | 0.201 | 0.097 0.209 93.75 pg/ml | 0.158 | 0.149 | 0.045 0.14 0 pg/ml | 0.106 | 0.104 | 0 0.102

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 349 | 16.2 | 4.6 Intra-assay | 2 | 20 | 759 | 15.4 | 2 Intra-assay | 3 | 20 | 1651 | 40.3 | 2.4 Inter-assay | 1 | 20 | 352 | 24.2 | 6.9 Inter-assay | 2 | 20 | 756 | 28.4 | 3.8 Inter-assay | 3 | 20 | 1639 | 66.9 | 4.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail