Human Beta-2-Glycoprotein 1, APOH ELISA Kit

Apolipoprotein H (APOH), also known as Beta-2-glycoprotein 1, Activated protein C-binding protein, B2GPI, and B2G1, is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. It is an essential cofactor for the binding of certain antiphospholipid antibodies (APA) to anionic phospholipid. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. APOH may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. APOH appears to completely inhibit serotonin release by the platelets and prevents subsequent waves of the ADP-induced aggregation. The activity of APOH appears to involve the binding of agglutenating, negatively charged compounds, and inhibits agglutenation by the contact activation of the intrinsic blood coagulation pathway. APOH causes a reduction of the prothrombinase binding sites on platelets and reduces the activation caused by collagen when thrombin is present at physiological serum concentrations of APOH suggesting a regulatory role of APOH in coagulation. APOH plasma concentrations are strongly associated to metabolic syndrome alterations and vascular disease in type 2 diabetic and could be considered as a clinical marker of cardiovascular risk. APOH is found on several classes of lipoproteins, and is involved in the activation of lipoprotein lipase in lipid metabolism. This single-chain glycoprotein also has been implicated in several physiologic pathways including coagulation and the production of hypertension, which are related to the pathogenesis of primary cerebral hemorrhage (PICH) .

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.519 | 2.586 | 2.47 2.652 2000 pg/ml | 1.401 | 1.465 | 1.349 1.529 1000 pg/ml | 0.968 | 0.938 | 0.822 0.908 500 pg/ml | 0.501 | 0.531 | 0.415 0.561 250 pg/ml | 0.309 | 0.311 | 0.195 0.313 125 pg/ml | 0.201 | 0.204 | 0.088 0.207 62.5 pg/ml | 0.144 | 0.151 | 0.035 0.157 0 pg/ml | 0.113 | 0.116 | 0 0.118

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 262 | 13 | 5 Intra-assay | 2 | 20 | 582 | 19.1 | 3.3 Intra-assay | 3 | 20 | 2326 | 86.7 | 3.7 Inter-assay | 1 | 20 | 264 | 24 | 9.1 Inter-assay | 2 | 20 | 588 | 38.6 | 6.6 Inter-assay | 3 | 20 | 2315 | 126 | 5.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail