Rat Leukemia Inhibitory Factor Receptor, Lifr ELISA Kit

LIFR (leukemia inhibitory factor receptor) belongs to the family of cytokine receptors. LIFR forms a high-affinity receptor complex with gp130, which mediates the activity of LIF (leukemia inhibitory factor) and thus affects the differentiation, proliferation, and survival of a wide variety of cells in the adult and the embryo. Besides LIF, LIFR can also bind to and activate CNTF (ciliary neurotrophic factor) and CLC (Cardiotrophin Like Cytokine) . Evidence showed that in the retina, LIFR activating LIF, CT-1, and Cardiotrophin Like Cytokine (CLC) are strongly upregulated in response to preconditioning with bright cyclic light leading to robust activation of signal transducer and activator of transcription-3 (STAT3) in a time-dependent manner. Further, blocking LIFR activation during preconditioning using a LIFR antagonist (LIF05) attenuated the induced STAT3 activation and also resulted in reduced preconditioning-induced protection of the retinal photoreceptors. These data demonstrate that LIFR and its ligands play an essential role in endogenous neuroprotective mechanisms triggered by preconditioning-induced stress. LIFR was newly found to be a suppressor of hepatocellular carcinoma (HCC), one of the world's top five causes of cancer-related deaths.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.522 | 2.651 | 2.565 2.781 1250 pg/ml | 1.564 | 1.534 | 1.448 1.505 625 pg/ml | 0.855 | 0.846 | 0.76 0.838 312.5 pg/ml | 0.453 | 0.486 | 0.4 0.52 156.25 pg/ml | 0.294 | 0.288 | 0.202 0.283 78.125 pg/ml | 0.183 | 0.188 | 0.102 0.194 39.0625 pg/ml | 0.127 | 0.133 | 0.047 0.14 0 pg/ml | 0.092 | 0.086 | 0 0.081

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 179 | 4.6 | 2.6 Intra-assay | 2 | 20 | 448 | 17.5 | 3.9 Intra-assay | 3 | 20 | 1950 | 78 | 4 Inter-assay | 1 | 20 | 177 | 14.4 | 8.1 Inter-assay | 2 | 20 | 444 | 36.1 | 8.1 Inter-assay | 3 | 20 | 1940 | 116 | 6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail