Human Pentraxin-Related Protein Ptx3, PTX3 ELISA Kit

Pentraxin-related protein PTX3, also known as Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein, TSG-14, PTX3 and TNFAIP5, is a secreted protein that contains one pentaxin domain. PTX3 plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. Pentraxins are a family of evolutionarily conserved multifunctional pattern-recognition proteins characterized by a cyclic multimeric structure. Based on the primary structure of the subunit, the pentraxins are divided into two groups: short pentraxins and long pentraxins. C-reactive protein (CRP) and serum amyloid P-component (SAP) are the two short pentraxins. The prototype protein of the long pentraxin group is pentraxin 3 (PTX3) . CRP and SAP are produced primarily in the liver in response to IL-6, while PTX3 is produced by a variety of tissues and cells and in particular by innate immunity cells in response to proinflammatory signals and Toll-like receptor (TLR) engagement. PTX3 is essential in female fertility by acting as a nodal point for the assembly of the cumulus oophorus hyaluronan-rich extracellular matrix. PTX3 interacts with several ligands, including growth factors, extracellular matrix components and selected pathogens, playing a role in complement activation and facilitating pathogen recognition by phagocytes, acting as a predecessor of antibodies. PTX3 may also contribute to the pathogenesis of atherosclerosis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.499 | 2.415 | 2.319 2.33 1250 pg/ml | 1.441 | 1.432 | 1.336 1.423 625 pg/ml | 0.729 | 0.773 | 0.677 0.817 312.5 pg/ml | 0.435 | 0.436 | 0.34 0.436 156.25 pg/ml | 0.255 | 0.258 | 0.162 0.261 78.125 pg/ml | 0.178 | 0.168 | 0.072 0.158 39.0625 pg/ml | 0.137 | 0.134 | 0.038 0.13 0 pg/ml | 0.098 | 0.096 | 0 0.093

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 321 | 23.9 | 7.5 Intra-assay | 2 | 20 | 698 | 40.8 | 5.9 Intra-assay | 3 | 20 | 1789 | 70.4 | 3.9 Inter-assay | 1 | 20 | 320 | 16.6 | 5.2 Inter-assay | 2 | 20 | 699 | 36.4 | 5.2 Inter-assay | 3 | 20 | 1784 | 91 | 5.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail