Mouse C-Reactive Protein, Crp ELISA Kit

C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2200 pg/ml | 2.124 | 2.192 | 2.078 2.26 1100 pg/ml | 1.195 | 1.244 | 1.13 1.292 550 pg/ml | 0.655 | 0.685 | 0.571 0.715 275 pg/ml | 0.424 | 0.403 | 0.289 0.381 137.5 pg/ml | 0.245 | 0.262 | 0.148 0.279 68.75 pg/ml | 0.177 | 0.178 | 0.064 0.179 34.375 pg/ml | 0.149 | 0.15 | 0.036 0.15 0 pg/ml | 0.108 | 0.114 | 0 0.119

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 148 | 12.9 | 8.7 Intra-assay | 2 | 20 | 436 | 32.8 | 7.5 Intra-assay | 3 | 20 | 1557 | 128 | 8.2 Inter-assay | 1 | 20 | 149 | 10.9 | 7.3 Inter-assay | 2 | 20 | 438 | 35 | 8 Inter-assay | 3 | 20 | 1562 | 106 | 6.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail