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Human Neural Cell Adhesion Molecule L1-Like Protein, CHL1 ELISA Kit

Product Specifications

Background

Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin type III domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved in hemophilic disease.

Product Name Alternative

Neural cell adhesion molecule L1-like protein

Synonyms

CALL; FLJ44930; L1CAM2; MGC132578

Gene Name

CHL1

Gene ID

10752

UniProt

O00533

Target

CHL1

Field of Research

Neuroscience, Signal transduction

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 1.71 | 1.715 | 1.633 1.72 1000 pg/ml | 1.133 | 1.183 | 1.101 1.232 500 pg/ml | 0.76 | 0.741 | 0.659 0.721 250 pg/ml | 0.446 | 0.432 | 0.35 0.417 125 pg/ml | 0.248 | 0.261 | 0.179 0.273 62.5 pg/ml | 0.17 | 0.171 | 0.089 0.171 31.25 pg/ml | 0.119 | 0.123 | 0.041 0.126 0 pg/ml | 0.078 | 0.082 | 0 0.086

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

31.25-2000 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 311 | 24 | 7.7 Intra-assay | 2 | 20 | 707 | 31.9 | 4.5 Intra-assay | 3 | 20 | 1413 | 78.9 | 5.6 Inter-assay | 1 | 20 | 308 | 27 | 8.8 Inter-assay | 2 | 20 | 712 | 63.8 | 9 Inter-assay | 3 | 20 | 1411 | 118 | 8.4

Sensitivity

10.21 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

135071

Available Sizes

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