Human Alpha-2-Antiplasmin, SERPINF2 ELISA Kit

SerpinF2, also known as alpha-2 antiplasmin (alpha-2 AP), is a member of the Serpin superfamily. SerpinF2 is the principal physiological inhibitor of serine protease plasmin, and as well as, an efficient inhibitor of trypsin and chymotrypsin. This protease is produced mainly by liver and kidney, and also expressed in muscle, intestine, central nervous system, and placenta at a moderate level. It is indicated that Serpin F2 is a key regulator of plasmin-mediated proteolysis in these tissues. Alpha-2 AP is an unusual serpin in that it contains extensive N- and C-terminal sequences flanking the serpin domain. The N-terminal sequence is crosslinked to fibrin by factor XIIIa, whereas the C-terminal region mediates the initial interaction with plasmin. SerpinF2 is one of the inhibitors of fibrinolysis, which acts as the primary inhibitor of plasmin (ogen) . It is a specific plasmin inhibitor, and is important in modulating the effectiveness and persistence of fibrin with respect to its susceptibility to digestion and removal by plasmin. Alpha-2 AP plays the dominant role in inhibiting both plasma clot lysis and thrombus lysis, and accordingly, the congenital deficiency of Alpha-2 antiplasmin causes a rare bleeding disorder because of increased fibrinolysis. Thus, it may be a useful target for developing more effective treatment of thrombotic diseases.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.048 | 2.14 | 2.056 2.233 1000 pg/ml | 1.089 | 1.13 | 1.046 1.171 500 pg/ml | 0.64 | 0.61 | 0.526 0.581 250 pg/ml | 0.353 | 0.349 | 0.265 0.346 125 pg/ml | 0.206 | 0.217 | 0.133 0.229 62.5 pg/ml | 0.153 | 0.15 | 0.066 0.148 31.25 pg/ml | 0.122 | 0.114 | 0.03 0.107 0 pg/ml | 0.084 | 0.084 | 0 0.085

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 68.3 | 3.7 | 5.4 Intra-assay | 2 | 20 | 342 | 14.4 | 4.2 Intra-assay | 3 | 20 | 1424 | 92.9 | 6.5 Inter-assay | 1 | 20 | 68.6 | 7.5 | 11 Inter-assay | 2 | 20 | 344 | 27.7 | 8.1 Inter-assay | 3 | 20 | 1419 | 120 | 8.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail