Human Alpha-1-Antitrypsin, SERPINA1 ELISA Kit

SerpinA1, also known as Alpha-1 antitrypsin (AAT), is a prototype member of the Serpin superfamily of the serine protease inhibitors. This serine protease inhibitor blocks the protease, neutrophil elastase. Alpha-1 antitrypsin is mainly produced in the liver and acts as an antiprotease. Its principal function is to inactivate neutrophil elastase, preventing tissue damage. SerpinA1 (alpha1-antitrypsin), an acute phase protein and the classical neutrophil elastase inhibitor, is localized within lipid rafts in primary human monocytes in vitro. Its association with monocytes is inhibited by cholesterol depleting/efflux-stimulating agents (nystatin, filipin, MbetaCD (methyl-beta-cyclodextrin) and oxidized low-density lipoprotein (oxLDL) and conversely, enhanced by free cholesterol. Furthermore, SerpinA1/monocyte association per se depletes lipid raft cholesterol as characterized by the activation of extracellular signal-regulated kinase 2, formation of cytosolic lipid droplets, and complete inhibition of oxLDL uptake by monocytes. Previous population studies have suggested that heterozygote status for the AAT gene (SerpinA1) is a risk factor for chronic rhinosinusitis with nasal polyposis (CRSwNP) . Alpha-1 antitrypsin deficiency is a recently identified genetic disease that occurs almost as frequently as cystic fibrosis. It is caused by various mutations in the SerpinA1 gene, and has numerous clinical implications. Alpha-1 antitrypsin deficiency is an inherited disease affecting the lung and liver. In the liver, alpha-1 antitrypsin deficiency may manifest as benign neonatal hepatitis syndrome; a small percentage of adults develop liver fibrosis, with progression to cirrhosis and hepatocellular carcinoma. Its most important physiologic functions are the protection of pulmonary tissue from aggressive proteolytic enzymes and regulation of pulmonary immune processes.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.295 | 2.203 | 2.118 2.112 1000 pg/ml | 1.187 | 1.176 | 1.091 1.165 500 pg/ml | 0.65 | 0.637 | 0.552 0.624 250 pg/ml | 0.366 | 0.36 | 0.275 0.355 125 pg/ml | 0.228 | 0.223 | 0.138 0.218 62.5 pg/ml | 0.168 | 0.161 | 0.076 0.154 31.25 pg/ml | 0.125 | 0.125 | 0.04 0.126 0 pg/ml | 0.083 | 0.085 | 0 0.088

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 171 | 15.9 | 9.3 Intra-assay | 2 | 20 | 428 | 39 | 9.1 Intra-assay | 3 | 20 | 1584 | 79.1 | 5 Inter-assay | 1 | 20 | 170 | 16.4 | 9.7 Inter-assay | 2 | 20 | 426 | 36.1 | 8.5 Inter-assay | 3 | 20 | 1587 | 115 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail