Human Bone Morphogenetic Protein 2, BMP2 ELISA Kit

BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.326 | 2.262 | 2.178 2.198 1000 pg/ml | 1.208 | 1.263 | 1.179 1.318 500 pg/ml | 0.644 | 0.662 | 0.578 0.681 250 pg/ml | 0.412 | 0.399 | 0.315 0.387 125 pg/ml | 0.211 | 0.226 | 0.142 0.241 62.5 pg/ml | 0.149 | 0.145 | 0.061 0.142 31.25 pg/ml | 0.111 | 0.114 | 0.03 0.117 0 pg/ml | 0.088 | 0.084 | 0 0.08

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 160 | 7.4 | 4.6 Intra-assay | 2 | 20 | 550 | 25.1 | 4.6 Intra-assay | 3 | 20 | 1280 | 59.6 | 4.7 Inter-assay | 1 | 20 | 160 | 13.2 | 8.2 Inter-assay | 2 | 20 | 544 | 29.6 | 5.4 Inter-assay | 3 | 20 | 1289 | 64.7 | 5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail