Human Lysosome-Associated Membrane Glycoprotein 3, LAMP3 ELISA Kit

Dendritic cell-lysosomal associated membrane protein (DC-LAMP) /CD208, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as a marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm180, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in the activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.916 | 2.919 | 2.827 2.923 1000 pg/ml | 1.431 | 1.4 | 1.308 1.37 500 pg/ml | 0.891 | 0.871 | 0.779 0.852 250 pg/ml | 0.483 | 0.461 | 0.369 0.44 125 pg/ml | 0.264 | 0.256 | 0.164 0.249 62.5 pg/ml | 0.156 | 0.161 | 0.069 0.167 31.25 pg/ml | 0.123 | 0.126 | 0.034 0.13 0 pg/ml | 0.094 | 0.092 | 0 0.09

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 79 | 2.6 | 3.3 Intra-assay | 2 | 20 | 343 | 11.8 | 3.4 Intra-assay | 3 | 20 | 1561 | 54.9 | 3.5 Inter-assay | 1 | 20 | 79.9 | 8.1 | 10.1 Inter-assay | 2 | 20 | 342 | 24.5 | 7.2 Inter-assay | 3 | 20 | 1542 | 107 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail