Human Platelet-Derived Growth Factor Receptor Alpha, PDGFRA ELISA Kit

PDGFRA, also known as CD140a, together with the structurally homolog protein PDGFRB (CD140b), are cell surface receptors for members of the platelet-derived growth factor family. They are members of the class III subfamily of receptor tyrosine kinase (RTKs) with the similar structure characteristics of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGFRA is expressed in oligodendrocyte progenitor cells and mesothelial cell, and binds all three ligand isoforms PDGF-AA, PDGF-BB and PDGF-AB with high affinity, whereas PDGFRB dose not bind PDGF-AA. PDGFRA plays an essential role in regulating proliferation, chemotaxis and migration of mesangial cells. Recent studies have indicated that PDGFRA acts as a critical mediator of signaling in testis organogenesis and Leydig cell differentiation, and in addition, particularly important for kidney development. Additionally, PDGFRA is involved in tumor angiogenesis and maintenance of the tumor microenvironment and has been implicated in development and metastasis of Hepatocellular carcinoma (HCC) . PDGFRA may represent a potential therapeutic target in thymic tumours. PDGFRA gene amplification rather than gene mutation may be the underlying genetic mechanism driving PDGFRA overexpression in a portion of gliomas. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 1.524 | 1.518 | 1.405 1.512 1000 pg/ml | 0.934 | 0.967 | 0.854 1 500 pg/ml | 0.535 | 0.567 | 0.454 0.599 250 pg/ml | 0.349 | 0.351 | 0.238 0.353 125 pg/ml | 0.238 | 0.237 | 0.124 0.236 62.5 pg/ml | 0.166 | 0.176 | 0.063 0.186 31.25 pg/ml | 0.145 | 0.146 | 0.033 0.148 0 pg/ml | 0.111 | 0.113 | 0 0.115

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 236 | 12.4 | 5.3 Intra-assay | 2 | 20 | 525 | 16.9 | 3.2 Intra-assay | 3 | 20 | 1314 | 30.2 | 2.3 Inter-assay | 1 | 20 | 239 | 12.8 | 5.4 Inter-assay | 2 | 20 | 530 | 29.3 | 5.5 Inter-assay | 3 | 20 | 1317 | 65.1 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail