Human Interleukin-17 Receptor A, IL17RA ELISA Kit

Interleukin-17 receptor (IL-17R), also known as Interleukin-17 receptor A (IL-17RA) and CD217 antigen (CD217), is a cytokine receptor that binds interleukin 17. IL-17R/IL-17RA (CD217) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17R/IL-17RA (CD217) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor IL-17RA play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Defects in IL-17R/IL-17RA (CD217) are the cause of familial candidiasis type 5 (CANDF5) . CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails, and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.832 | 2.815 | 2.689 2.798 1000 pg/ml | 1.594 | 1.515 | 1.389 1.436 500 pg/ml | 0.855 | 0.823 | 0.697 0.791 250 pg/ml | 0.471 | 0.461 | 0.335 0.451 125 pg/ml | 0.278 | 0.297 | 0.171 0.316 62.5 pg/ml | 0.228 | 0.217 | 0.091 0.206 31.25 pg/ml | 0.164 | 0.172 | 0.046 0.18 0 pg/ml | 0.121 | 0.126 | 0 0.131

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 133 | 6.9 | 5.2 Intra-assay | 2 | 20 | 428 | 15.7 | 3.7 Intra-assay | 3 | 20 | 1188 | 36.6 | 3.1 Inter-assay | 1 | 20 | 133 | 11.6 | 8.7 Inter-assay | 2 | 20 | 426 | 41.4 | 9.7 Inter-assay | 3 | 20 | 1201 | 117 | 9.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail