Human Ficolin-1, FCN1 ELISA Kit

Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. The Ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins are humoral molecules of the innate immune systems which recognize carbohydrate molecules on pathogens, apoptotic and necrotic cells. Three Ficolins have been identified in humans: L-Ficolin, H-Ficolin and M-Ficolin (also referred to as Ficolin-2, -3 and -1, respectively) . They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Dysfunction or abnormal expressions of Ficolins may involved in the pathogenesis of human diseases including infectious and inflammatory diseases, autoimmune disease and clinical syndrome of preeclampsia. They are soluble oligomeric defence proteins with lectin-like activity and they are structurally similar to the human collectins, mannan-binding lectin (MBL) and surfactant protein A and D. Upon recognition of the infectious agent, the Ficolins act through two distinct routes: initiate the lectin pathway of complement activation through attached serine proteases (MASPs), and a primitive opsonophagocytosis thus limiting the infection and concurrently orchestrating the subsequent adaptive clonal immune response. Ficolin-1 (FCN1) is predominantly expressed in the peripheral blood leukocytes.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.021 | 2.072 | 1.962 2.124 1000 pg/ml | 1.259 | 1.263 | 1.153 1.268 500 pg/ml | 0.751 | 0.712 | 0.602 0.673 250 pg/ml | 0.409 | 0.412 | 0.302 0.416 125 pg/ml | 0.249 | 0.266 | 0.156 0.284 62.5 pg/ml | 0.196 | 0.194 | 0.084 0.192 31.25 pg/ml | 0.143 | 0.153 | 0.043 0.164 0 pg/ml | 0.112 | 0.11 | 0 0.109

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 166 | 13 | 7.8 Intra-assay | 2 | 20 | 353 | 17.7 | 5 Intra-assay | 3 | 20 | 1413 | 48.2 | 3.4 Inter-assay | 1 | 20 | 168 | 14.9 | 8.9 Inter-assay | 2 | 20 | 352 | 25 | 7.1 Inter-assay | 3 | 20 | 1427 | 87.1 | 6.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail