Human Interleukin-13 Receptor Subunit Alpha-1, IL13RA1 ELISA Kit

Interleukin 13 receptor, alpha 1, also known as IL13RA1/IL-13RA1 and CD213A1 (cluster of differentiation 213A1), is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. IL13RA1/IL-13RA1 serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2 and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3, and STAT6 induced by IL13 and IL4. IL13RA1/IL-13RA1 binds with low affinity to interleukin-13 (IL13) . This subunit together with IL4RA can form a functional receptor for IL13. IL13RA1/IL-13RA1 also serves as an alternate accessory protein to the common cytokine receptor gamma chain for interleukin-4 (IL4) signaling, but cannot replace the function of IL2RG in allowing enhanced interleukin-2 (IL2) binding activity.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 1.706 | 1.616 | 1.519 1.525 1000 pg/ml | 0.934 | 0.989 | 0.892 1.043 500 pg/ml | 0.579 | 0.599 | 0.502 0.618 250 pg/ml | 0.342 | 0.347 | 0.25 0.351 125 pg/ml | 0.214 | 0.223 | 0.126 0.231 62.5 pg/ml | 0.163 | 0.158 | 0.061 0.153 31.25 pg/ml | 0.132 | 0.125 | 0.028 0.117 0 pg/ml | 0.098 | 0.097 | 0 0.095

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 189 | 18.9 | 10 Intra-assay | 2 | 20 | 572 | 19.3 | 3.4 Intra-assay | 3 | 20 | 1301 | 45.2 | 3.5 Inter-assay | 1 | 20 | 188 | 18 | 9.6 Inter-assay | 2 | 20 | 567 | 44 | 7.8 Inter-assay | 3 | 20 | 1309 | 105 | 8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail