Human Calmodulin-Like Protein 5, CALML5 ELISA Kit

Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5 / CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5 / CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5 / CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5 / CLSP have been characterized by NMR spectroscopy. The mobility of CALML5 / CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.441 | 2.419 | 2.314 2.396 1000 pg/ml | 1.404 | 1.389 | 1.284 1.374 500 pg/ml | 0.697 | 0.726 | 0.621 0.754 250 pg/ml | 0.444 | 0.431 | 0.326 0.417 125 pg/ml | 0.253 | 0.269 | 0.164 0.284 62.5 pg/ml | 0.181 | 0.192 | 0.087 0.203 31.25 pg/ml | 0.137 | 0.139 | 0.034 0.14 0 pg/ml | 0.109 | 0.105 | 0 0.1

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 103 | 2.7 | 2.7 Intra-assay | 2 | 20 | 272 | 9.7 | 3.6 Intra-assay | 3 | 20 | 1131 | 31 | 2.7 Inter-assay | 1 | 20 | 102 | 4 | 3.9 Inter-assay | 2 | 20 | 270 | 20.2 | 7.5 Inter-assay | 3 | 20 | 1124 | 43.6 | 3.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail