Human V-Set And Immunoglobulin Domain-Containing Protein 4, VSIG4 ELISA Kit

VSIG4 (V-set and immunoglobulin domain containing 4), also known as complement receptor of the immunoglobulin superfamily (CRIg) and Z39Ig, is a type I transmembrane glycoprotein. It is a B7 family-related protein and an Ig superfamily member. In contrast to the B7 family members which contain two IgG domains, VSIG4 contains one complete V-type I g domain and a truncated C-type I g domain. VSIG4 is exclusively expressed on tissue resident macrophages and binds to multimers of C3b and iC3b that are covalently attached to particle surfaces. No VSIG4 expression appears to be present in T and B cells. VSIG4 functions as a negative regulator of T cell activation, and may be involved in the maintenance of peripheral T cell tolerance, and is also identified as a potent suppressor of established inflammation. Mouse VSIG4 is synthesized as a 28 amino acid precursor that contains a signal sequence, a V-type I g domain (aa 36-115), one potential N-linked glycosylation site, and a single transmembrane domain. The V-type I g domain of mouse VSIG4 shares 86% and 8% aa sequence identity with the V-type I g domains of rat and human VSIG4, respectively.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.424 | 2.386 | 2.284 2.348 1000 pg/ml | 1.254 | 1.257 | 1.155 1.26 500 pg/ml | 0.62 | 0.662 | 0.56 0.704 250 pg/ml | 0.391 | 0.38 | 0.278 0.37 125 pg/ml | 0.22 | 0.228 | 0.126 0.237 62.5 pg/ml | 0.162 | 0.162 | 0.06 0.163 31.25 pg/ml | 0.131 | 0.124 | 0.022 0.117 0 pg/ml | 0.097 | 0.102 | 0 0.108

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 169 | 7.3 | 4.3 Intra-assay | 2 | 20 | 651 | 22 | 3.4 Intra-assay | 3 | 20 | 1551 | 73.9 | 4.8 Inter-assay | 1 | 20 | 168 | 16.8 | 10 Inter-assay | 2 | 20 | 650 | 44.7 | 6.9 Inter-assay | 3 | 20 | 1541 | 43.9 | 2.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail