Human Complement Component C7, C7 ELISA Kit

Complement component 7 is a component of the complement system. It belongs to the complement C6/C7/C8/C9 family. It contains 1 EGF-like domain, 1 LDL-receptor class A domain, 1 MACPF domain, 2 Sushi (CCP/SCR) domains and 2 TSP type-1 domains. Complement component 7 serves as a membrane anchor. It participates in the formation of Membrane Attack Complex (MAC) . People with C7 deficiency are prone to bacterial infection. It is a constituent of MAC that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C7 are a cause of complement component 7 deficiency (C7D) . A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 4000 pg/ml | 2.904 | 2.811 | 2.714 2.719 2000 pg/ml | 1.423 | 1.483 | 1.386 1.544 1000 pg/ml | 0.815 | 0.793 | 0.696 0.772 500 pg/ml | 0.453 | 0.428 | 0.331 0.403 250 pg/ml | 0.268 | 0.26 | 0.163 0.253 125 pg/ml | 0.18 | 0.177 | 0.08 0.175 62.5 pg/ml | 0.129 | 0.137 | 0.04 0.146 0 pg/ml | 0.099 | 0.097 | 0 0.096

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 299 | 16.2 | 5.4 Intra-assay | 2 | 20 | 696 | 29.9 | 4.3 Intra-assay | 3 | 20 | 2108 | 136 | 6.5 Inter-assay | 1 | 20 | 299 | 32.5 | 10.9 Inter-assay | 2 | 20 | 689 | 54.7 | 7.9 Inter-assay | 3 | 20 | 2117 | 180 | 8.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail