Mouse Protein S100-A1, S100a1 ELISA Kit

S100A1 is a Ca2+ binding protein of the EF-hand type that belongs to the S100 protein family. S100 proteins consisting of at least 19 members exist as dimers in the cytoplasm and/or nucleus of a wide range of cells, and are involved in the regulation of a number of cellular processes such as cell-cycle progression and cell differentiation. This protein has been shown to function in the processes including stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of PKC-mediated phosphorylation. Phosphoglucomutase is a target protein whose activity is antagonistically regulated by S100A1, and recently, S100A1 is also identified as a potent molecular chaperone and a new member of the Hsp70/Hsp90 multichaperone complex. S100A1 displays a tissue-specific expression pattern with highest levels in myocardium and is considered to be an important regulator of cardiac contractility. Accordingly, reduced expression or mutations of S100A1 gene have been implicated in cardiomyopathies.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.808 | 2.792 | 2.688 2.775 1000 pg/ml | 1.486 | 1.478 | 1.374 1.469 500 pg/ml | 0.81 | 0.808 | 0.704 0.806 250 pg/ml | 0.454 | 0.464 | 0.36 0.473 125 pg/ml | 0.279 | 0.28 | 0.176 0.28 62.5 pg/ml | 0.194 | 0.192 | 0.088 0.189 31.25 pg/ml | 0.134 | 0.141 | 0.037 0.148 0 pg/ml | 0.103 | 0.104 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 90.4 | 3.5 | 3.9 Intra-assay | 2 | 20 | 266 | 17.8 | 6.7 Intra-assay | 3 | 20 | 1023 | 27.6 | 2.7 Inter-assay | 1 | 20 | 91.1 | 5 | 5.5 Inter-assay | 2 | 20 | 264 | 15.7 | 5.9 Inter-assay | 3 | 20 | 1026 | 47.1 | 4.6

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail