Rat Intercellular Adhesion Molecule 1, Icam1 ELISA Kit

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 3.2 | 3.12 | 3.016 3.041 1000 pg/ml | 1.787 | 1.727 | 1.623 1.668 500 pg/ml | 1.008 | 0.956 | 0.852 0.905 250 pg/ml | 0.556 | 0.52 | 0.416 0.485 125 pg/ml | 0.319 | 0.303 | 0.199 0.288 62.5 pg/ml | 0.2 | 0.199 | 0.095 0.199 31.25 pg/ml | 0.162 | 0.152 | 0.048 0.143 0 pg/ml | 0.105 | 0.104 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 237 | 6.1 | 2.6 Intra-assay | 2 | 20 | 593 | 22.4 | 3.8 Intra-assay | 3 | 20 | 1348 | 52.4 | 3.9 Inter-assay | 1 | 20 | 236 | 19.4 | 8.2 Inter-assay | 2 | 20 | 594 | 47.3 | 8 Inter-assay | 3 | 20 | 1358 | 80.3 | 5.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail