Human Complement Receptor Type 2, CR2 ELISA Kit

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD21, also known as Complement component (3d / Epstein Barr virus) receptor 2 and CR2, is a member of the CD system and is a protein involved in complement system. CD21 is present on all mature B-cells and some T-cells and follicular dendritic cells. CD21 on mature B-cells form a complex called the B cell receptor complex with two other membrane proteins, CD19 and CD81. CD21 has a function in the complement system through serving as the cellular receptor specific for ligands such as C3 and C4 which can be attached to foreign macromolecules in order to remove or uptake them. This results in B-cells having enhanced response to the antigen.

Concentration | O.D. | Average | Corrected ------------------------------------------ 2000 pg/ml | 2.389 | 2.484 | 2.389 2.58 1000 pg/ml | 1.404 | 1.36 | 1.265 1.316 500 pg/ml | 0.74 | 0.789 | 0.694 0.839 250 pg/ml | 0.469 | 0.445 | 0.35 0.422 125 pg/ml | 0.275 | 0.269 | 0.174 0.264 62.5 pg/ml | 0.176 | 0.185 | 0.09 0.194 31.25 pg/ml | 0.14 | 0.138 | 0.043 0.137 0 pg/ml | 0.099 | 0.095 | 0 0.091

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 317 | 11.7 | 3.7 Intra-assay | 2 | 20 | 738 | 23.6 | 3.2 Intra-assay | 3 | 20 | 1570 | 45.9 | 2.9 Inter-assay | 1 | 20 | 317 | 26.1 | 8.2 Inter-assay | 2 | 20 | 743 | 60.3 | 8.1 Inter-assay | 3 | 20 | 1578 | 77.1 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail