Human Interleukin-12 ELISA Kit

Concentration | O.D. | Average | Corrected ------------------------------------------ 2500 pg/ml | 2.283 | 2.345 | 2.244 2.408 1250 pg/ml | 1.198 | 1.18 | 1.079 1.163 625 pg/ml | 0.668 | 0.635 | 0.534 0.603 312.5 pg/ml | 0.333 | 0.355 | 0.254 0.378 156.25 pg/ml | 0.239 | 0.224 | 0.123 0.21 78.125 pg/ml | 0.16 | 0.163 | 0.062 0.167 39.0625 pg/ml | 0.134 | 0.132 | 0.031 0.131 0 pg/ml | 0.1 | 0.101 | 0 0.103

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 160 | 5.9 | 3.7 Intra-assay | 2 | 20 | 517 | 16 | 3.1 Intra-assay | 3 | 20 | 1203 | 63.8 | 5.3 Inter-assay | 1 | 20 | 161 | 13.3 | 8.3 Inter-assay | 2 | 20 | 513 | 31.8 | 6.2 Inter-assay | 3 | 20 | 1189 | 83.5 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail