Human Vascular Endothelial Growth Factor Receptor 2, KDR ELISA Kit

VEGFR2 also called KDR or Flk-1, is identified as the receptor for VEGF and VEGFC and an early marker for endothelial cell progenitors, whose expression is restricted to endothelial cells in vivo. VEGFR2 was shown to be the primary signal transducer for angiogenesis and the development of pathological conditions such as cancer and diabetic retinopathy. It has been shown that VEGFR2 is expressed mainly in the endothelial cells, and the expression is upregulated in the tumor vasculature. Thus the inhibition of VEGFR2 activity and its downstream signaling are important targets for the treatment of diseases involving angiogenesis. VEGFR2 transduces the major signals for angiogenesis via its strong tyrosine kinase activity. However, unlike other representative tyrosine kinase receptors, VEGFR2 does not use the Ras pathway as major downstream signaling but rather uses the phospholipase C-protein kinase C pathway to signal mitogen-activated protein (MAP) -kinase activation and DNA synthesis. VEGFR2 is a direct and major signal transducer for pathological angiogenesis, including cancer and diabetic retinopathy, in cooperation with many other signaling partners; thus, VEGFR2 and its downstream signaling appear to be critical targets for the suppression of these diseases. VEGF and VEGFR2-mediated survival signaling are critical to endothelial cell survival, maintenance of the vasculature and alveolar structure, and regeneration of lung tissue. Reduced VEGF and VEGFR2 expression in emphysematous lungs has been linked to increased endothelial cell death and vascular regression. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1600 pg/ml | 2.583 | 2.483 | 2.378 2.382 800 pg/ml | 1.513 | 1.474 | 1.369 1.435 400 pg/ml | 0.841 | 0.829 | 0.724 0.816 200 pg/ml | 0.473 | 0.467 | 0.362 0.461 100 pg/ml | 0.297 | 0.28 | 0.175 0.263 50 pg/ml | 0.193 | 0.186 | 0.081 0.178 25 pg/ml | 0.16 | 0.154 | 0.049 0.148 0 pg/ml | 0.104 | 0.105 | 0 0.106

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 118 | 6.2 | 5.3 Intra-assay | 2 | 20 | 303 | 10.1 | 3.3 Intra-assay | 3 | 20 | 977 | 22.8 | 2.3 Inter-assay | 1 | 20 | 117 | 6.5 | 5.5 Inter-assay | 2 | 20 | 305 | 17 | 5.6 Inter-assay | 3 | 20 | 974 | 47.4 | 4.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail