Human Cadherin-1, CDH1 ELISA Kit

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1600 pg/ml | 2.326 | 2.32 | 2.216 2.313 800 pg/ml | 1.413 | 1.357 | 1.253 1.3 400 pg/ml | 0.711 | 0.759 | 0.655 0.806 200 pg/ml | 0.438 | 0.437 | 0.333 0.435 100 pg/ml | 0.272 | 0.274 | 0.17 0.275 50 pg/ml | 0.179 | 0.19 | 0.086 0.2 25 pg/ml | 0.15 | 0.147 | 0.043 0.143 0 pg/ml | 0.103 | 0.104 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 111 | 5.5 | 5 Intra-assay | 2 | 20 | 347 | 14 | 4 Intra-assay | 3 | 20 | 827 | 36.1 | 4.4 Inter-assay | 1 | 20 | 110 | 11.4 | 10.4 Inter-assay | 2 | 20 | 346 | 18.6 | 5.4 Inter-assay | 3 | 20 | 835 | 35.9 | 4.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail