Human Programmed Cell Death 1 Ligand 2, PDCD1LG2 ELISA Kit

Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1. Cancer Immunotherapy Co-inhibitory Immune Checkpoint Targets Immune Checkpoint Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies Immune Checkpoint Proteins Immune Checkpoint Targets Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1600 pg/ml | 2.372 | 2.303 | 2.202 2.233 800 pg/ml | 1.248 | 1.28 | 1.179 1.312 400 pg/ml | 0.729 | 0.696 | 0.595 0.662 200 pg/ml | 0.359 | 0.386 | 0.285 0.412 100 pg/ml | 0.244 | 0.246 | 0.145 0.247 50 pg/ml | 0.178 | 0.171 | 0.07 0.163 25 pg/ml | 0.143 | 0.136 | 0.035 0.128 0 pg/ml | 0.107 | 0.101 | 0 0.094

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 75.3 | 5.4 | 7.1 Intra-assay | 2 | 20 | 188 | 8.2 | 4.3 Intra-assay | 3 | 20 | 941 | 35.8 | 3.8 Inter-assay | 1 | 20 | 75.5 | 4.8 | 6.4 Inter-assay | 2 | 20 | 186 | 17.2 | 9.2 Inter-assay | 3 | 20 | 947 | 76.4 | 8.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail