Human Bone marrow stromal antigen 1, Bst1 ELISA Kit

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD157, also known as ADP-ribosyl cyclase 2, is an ectoenzyme sharing several characteristics with ADP-ribosyl cyclase CD38. CD157 was originally identified as a bone marrow stromal cell molecule (BST-1) with a glycosylphosphatidylinositol (GPI) anchor to bind to the cell surface. CD157 is prevalently expressed by cells of the myeloid lineage. CD157 could act as a receptor with signal transduction capability. Further, it regulates calcium homeostasis and promotes polarization in neutrophils and mediates superoxide (O2−) production in the human U937 myeloid line.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.188 | 2.271 | 2.184 2.354 750 pg/ml | 1.299 | 1.295 | 1.208 1.292 375 pg/ml | 0.699 | 0.732 | 0.645 0.766 187.5 pg/ml | 0.424 | 0.418 | 0.331 0.412 93.75 pg/ml | 0.242 | 0.258 | 0.171 0.275 46.875 pg/ml | 0.174 | 0.171 | 0.084 0.169 23.4375 pg/ml | 0.142 | 0.136 | 0.049 0.13 0 pg/ml | 0.09 | 0.087 | 0 0.085

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 261 | 17.5 | 6.7 Intra-assay | 2 | 20 | 556 | 15.9 | 2.9 Intra-assay | 3 | 20 | 1135 | 29.4 | 2.6 Inter-assay | 1 | 20 | 262 | 26 | 9.9 Inter-assay | 2 | 20 | 560 | 41.9 | 7.5 Inter-assay | 3 | 20 | 1124 | 69.7 | 6.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail