Human Retinol-Binding Protein 4, RBP4 ELISA Kit

Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a β-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM) .

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.324 | 2.384 | 2.298 2.443 750 pg/ml | 1.398 | 1.355 | 1.269 1.312 375 pg/ml | 0.725 | 0.752 | 0.666 0.778 187.5 pg/ml | 0.421 | 0.407 | 0.321 0.393 93.75 pg/ml | 0.268 | 0.253 | 0.167 0.237 46.875 pg/ml | 0.176 | 0.174 | 0.088 0.172 23.4375 pg/ml | 0.133 | 0.134 | 0.048 0.134 0 pg/ml | 0.082 | 0.086 | 0 0.09

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 142 | 4.6 | 3.3 Intra-assay | 2 | 20 | 407 | 11.1 | 2.7 Intra-assay | 3 | 20 | 1100 | 26.4 | 2.4 Inter-assay | 1 | 20 | 142 | 8.8 | 6.2 Inter-assay | 2 | 20 | 411 | 19.7 | 4.8 Inter-assay | 3 | 20 | 1104 | 52.9 | 4.8

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail