Human Triggering Receptor Expressed On Myeloid Cells 1, TREM1 ELISA Kit

TREM1 (triggering receptor expressed on myeloid cells) is a type I transmembrane protein with a single Ig-like domain, and is selectively expressed on blood neutrophils and a subset of monocytes. As a member of the growing family of receptors related to NK cell receptors, TREM1 activates downstream signaling events with the help of an adapter protein called DAP12. Expression of TREM1 is up-regulated by bacterial LPS, a ligand for TLR4, as well as lipoteichoic acid. Although its natural ligand has not been identified, engagement of TREM1 with agonist mAbs triggers secretion of the proinflammatory cytokines TNF-α and IL-1β, as well as chemokines such as IL-8 and monocyte chemoattractant protein (MCP) -1. Intracellularly, TREM1 induces Ca2+ mobilization and tyrosine phosphorylation of extracellular signal-related kinase 1 (ERK1), ERK2 and phospholipase C-γ. In an animal model of LPS-induced septic shock, blockade of TREM1 signaling inhibited hyperresponsiveness and death. Thus, it has been demonstrated that TREM1 performs a critical function in immune responses involved in host defense against microbial challenges, and is suggested to be a potential therapeutic target for septic shock.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.542 | 2.668 | 2.568 2.793 750 pg/ml | 0.917 | 0.935 | 0.835 0.952 375 pg/ml | 0.364 | 0.349 | 0.249 0.334 187.5 pg/ml | 0.186 | 0.179 | 0.079 0.171 93.75 pg/ml | 0.127 | 0.129 | 0.029 0.131 46.875 pg/ml | 0.111 | 0.118 | 0.018 0.125 23.4375 pg/ml | 0.099 | 0.101 | 0.001 0.103 0 pg/ml | 0.096 | 0.1 | 0 0.104

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 119 | 9.2 | 7.7 Intra-assay | 2 | 20 | 253 | 13.7 | 5.4 Intra-assay | 3 | 20 | 1056 | 43.9 | 4.2 Inter-assay | 1 | 20 | 120 | 12.8 | 10.7 Inter-assay | 2 | 20 | 254 | 21.5 | 8.5 Inter-assay | 3 | 20 | 1048 | 73.3 | 7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail