Human Interleukin-32, IL32 ELISA Kit

IL-32 is a recently discovered cytokine that induces various proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6) and chemokines in both human and mouse cells through the NF-kappaB and p38 MAPK inflammatory signal pathways. It is regulated robustly by other major proinflammatory cytokines and is crucial to inflammation and immune responses. Four of the IL-32 isoforms (alpha, beta, gamma, and delta) are the most representative IL-32 transcripts, and the gamma isoform of IL-32 is the most active, although all isoforms are biologically active. IL-32, a cytokine produced mainly by T, natural killer, and epithelial cells induces significant amounts of TNFalpha and MIP-2 and increases the production of both cytokines in a dose-dependent manner. IL-32 has been implicated in inflammatory disorders, Mycobacterium tuberculosis infections, inflammatory bowel disease, and influenza A virus infection, as well as in some autoimmune diseases, such as rheumatoid arthritis, ulcerative colitis, and in the human stomach cancer, human lung cancer, and breast cancer tissues. Thus, IL-32 expression might be valuable as a biomarker for cancer.

NK4; TAIF; OTTHUMP00000236040; OTTHUMP00000236041; OTTHUMP00000236042; OTTHUMP00000236043; OTTHUMP00000236044; OTTHUMP00000236045; OTTHUMP00000236046; OTTHUMP00000236047; OTTHUMP00000236048; OTTHUMP00000236049; OTTHUMP00000236050; OTTHUMP00000236051; OTTHUMP00000236052; OTTHUMP00000236053; OTTHUMP00000236054; OTTHUMP00000241545; OTTHUMP00000241602; OTTHUMP00000241603; OTTHUMP00000241604; OTTHUMP00000241605; OTTHUMP00000241606; OTTHUMP00000241607; OTTHUMP00000241608; OTTHUMP00000241609; OTTHUMP00000241610; OTTHUMP00000241611; OTTHUMP00000241612; TAIFa; TAIFb; TAIFc; TAIFd

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.139 | 2.226 | 2.113 2.313 750 pg/ml | 1.038 | 1.078 | 0.965 1.117 375 pg/ml | 0.528 | 0.565 | 0.452 0.602 187.5 pg/ml | 0.328 | 0.324 | 0.211 0.32 93.75 pg/ml | 0.195 | 0.199 | 0.086 0.203 46.875 pg/ml | 0.152 | 0.143 | 0.03 0.133 23.4375 pg/ml | 0.123 | 0.116 | 0.003 0.109 0 pg/ml | 0.109 | 0.113 | 0 0.117

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 112 | 3.1 | 2.8 Intra-assay | 2 | 20 | 430 | 9.1 | 2.1 Intra-assay | 3 | 20 | 1075 | 34.9 | 3.2 Inter-assay | 1 | 20 | 111 | 7.8 | 7 Inter-assay | 2 | 20 | 427 | 28.5 | 6.7 Inter-assay | 3 | 20 | 1087 | 73.3 | 6.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail