Human Nucleoside Diphosphate Phosphatase Entpd5, ENTPD5 ELISA Kit

Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.443 | 2.535 | 2.423 2.626 750 pg/ml | 1.381 | 1.387 | 1.275 1.392 375 pg/ml | 0.777 | 0.757 | 0.645 0.737 187.5 pg/ml | 0.421 | 0.443 | 0.331 0.464 93.75 pg/ml | 0.284 | 0.281 | 0.169 0.278 46.875 pg/ml | 0.194 | 0.197 | 0.085 0.199 23.4375 pg/ml | 0.161 | 0.154 | 0.042 0.147 0 pg/ml | 0.114 | 0.112 | 0 0.11

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 72.9 | 2.1 | 2.8 Intra-assay | 2 | 20 | 208 | 4.2 | 2 Intra-assay | 3 | 20 | 1042 | 33.3 | 3.2 Inter-assay | 1 | 20 | 73.6 | 5.2 | 7.1 Inter-assay | 2 | 20 | 206 | 14.1 | 6.8 Inter-assay | 3 | 20 | 1031 | 68.8 | 6.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail