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Human C-Type Lectin Domain Family 4 Member D, CLEC4D ELISA Kit

Product Specifications

Background

C-type lectin (CLEC) family is a type of carbohydrate-binding protein domain named lectin. C-type lectins are the most diverse and prevalent lectin family in immunity with its requirement for calcium for binding. Proteins including a C-type lectin domain have diverse range of functions including cell-cell adhesion, immune response to pathogens and apoptosis. There are at least 14 types of C-type lectins: typeIto typeXIV. CLEC4D (CLECSF8) is a typeII membrane glycoprotein belonging to the C-type lectin family, with restricted expression in the monocyte/macrophage lineage. It plays important roles in the function of macrophages.

Product Name Alternative

C-type lectin domain family 4 member D

Synonyms

CLECSF8; MCL; CLEC6; MGC40078; MPCL

Gene Name

CLEC4D

Gene ID

338339

UniProt

Q8WXI8

Target

CLEC4D

Field of Research

Immunology

Assay Type

Sandwich

Assay Protocol

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.398 | 2.422 | 2.305 2.446 750 pg/ml | 1.168 | 1.218 | 1.101 1.267 375 pg/ml | 0.689 | 0.662 | 0.545 0.635 187.5 pg/ml | 0.373 | 0.383 | 0.266 0.393 93.75 pg/ml | 0.241 | 0.238 | 0.121 0.234 46.875 pg/ml | 0.173 | 0.181 | 0.064 0.188 23.4375 pg/ml | 0.152 | 0.15 | 0.033 0.148 0 pg/ml | 0.119 | 0.117 | 0 0.115

Assay Performance Time

3.5h

Sample Type

Serum, plasma, cell culture supernates

Detection Range

23.44-1500 pg/mL

Precision

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 175 | 10.6 | 6 Intra-assay | 2 | 20 | 372 | 13.4 | 3.6 Intra-assay | 3 | 20 | 1033 | 45.5 | 4.4 Inter-assay | 1 | 20 | 173 | 16.2 | 9.4 Inter-assay | 2 | 20 | 368 | 18 | 4.9 Inter-assay | 3 | 20 | 1034 | 47.7 | 4.6

Sensitivity

5.49 pg/mL

Components

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail

Storage Temperature

2-8°C

Species

Human

Mass

24704

Available Sizes

Frequently Asked Questions

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