Human Cystatin-Sn, CST1 ELISA Kit

Cystatin-SN, also known as Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1 and CST1, is a secreted protein which belongs to thecystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. Cystatin-SN / CST1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level) . Cystatin-SN / CST1 is also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Human salivary cystatins include Cystatin-S, Cystatin-S1, Cystatin-S2, Cystatin- SA, Cystatin-SN, Cystatin-C and Cystatin-D. Cystatin-SN is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin-S, although both inhibit ficin equally well.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.198 | 2.306 | 2.215 2.414 750 pg/ml | 1.377 | 1.327 | 1.236 1.277 375 pg/ml | 0.71 | 0.747 | 0.656 0.784 187.5 pg/ml | 0.409 | 0.413 | 0.322 0.417 93.75 pg/ml | 0.252 | 0.248 | 0.157 0.244 46.875 pg/ml | 0.17 | 0.165 | 0.074 0.16 23.4375 pg/ml | 0.123 | 0.13 | 0.039 0.136 0 pg/ml | 0.089 | 0.091 | 0 0.093

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 123 | 5.9 | 4.8 Intra-assay | 2 | 20 | 245 | 11.7 | 4.8 Intra-assay | 3 | 20 | 1169 | 49.8 | 4.3 Inter-assay | 1 | 20 | 122 | 7.9 | 6.5 Inter-assay | 2 | 20 | 247 | 15.5 | 6.3 Inter-assay | 3 | 20 | 1177 | 72.3 | 6.1

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail