Human Macrophage Colony-Stimulating Factor 1, CSF1 ELISA Kit

Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.257 | 2.232 | 2.114 2.208 750 pg/ml | 1.242 | 1.232 | 1.114 1.222 375 pg/ml | 0.695 | 0.682 | 0.564 0.67 187.5 pg/ml | 0.406 | 0.413 | 0.295 0.42 93.75 pg/ml | 0.26 | 0.263 | 0.145 0.267 46.875 pg/ml | 0.189 | 0.198 | 0.08 0.208 23.4375 pg/ml | 0.165 | 0.161 | 0.043 0.158 0 pg/ml | 0.115 | 0.118 | 0 0.121

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 64.8 | 4.8 | 7.4 Intra-assay | 2 | 20 | 158 | 6.5 | 4.1 Intra-assay | 3 | 20 | 791 | 30.5 | 3.9 Inter-assay | 1 | 20 | 64.4 | 4.5 | 7 Inter-assay | 2 | 20 | 157 | 11.1 | 7.1 Inter-assay | 3 | 20 | 790 | 57.3 | 7.3

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail