Human Vasorin, VASN ELISA Kit

Vasorin is a typical type I membrane protein. It contains 1 EGF-like domain, 1 fibronectin type-III domain, 10 LRR (leucine-rich) repeats, 1 LRRCT domain and 1 LRRNT domain. Vasorin is predominantly expressed in vascular smooth muscle cells, and that its expression is developmentally regulated. vasorin It directly binds to transforming growth factor (TGF) -β and attenuates TGF-β signaling in vitro. This suggests that down-regulation of vasorin expression contributes to neointimal formation after vascular injury and that vasorin modulates cellular responses to pathological stimuli in the vessel wall.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.717 | 2.611 | 2.486 2.506 750 pg/ml | 1.294 | 1.355 | 1.23 1.416 375 pg/ml | 0.695 | 0.732 | 0.607 0.769 187.5 pg/ml | 0.401 | 0.418 | 0.293 0.436 93.75 pg/ml | 0.283 | 0.276 | 0.151 0.269 46.875 pg/ml | 0.191 | 0.2 | 0.075 0.21 23.4375 pg/ml | 0.17 | 0.167 | 0.042 0.165 0 pg/ml | 0.119 | 0.125 | 0 0.131

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 130 | 6.2 | 4.8 Intra-assay | 2 | 20 | 384 | 19.1 | 5 Intra-assay | 3 | 20 | 959 | 43.8 | 4.6 Inter-assay | 1 | 20 | 129 | 10.7 | 8.3 Inter-assay | 2 | 20 | 388 | 33.7 | 8.7 Inter-assay | 3 | 20 | 952 | 70.6 | 7.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail