Mouse Tissue Factor Pathway Inhibitor, Tfpi ELISA Kit

Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.311 | 2.331 | 2.237 2.351 750 pg/ml | 1.28 | 1.262 | 1.168 1.244 375 pg/ml | 0.743 | 0.695 | 0.601 0.647 187.5 pg/ml | 0.394 | 0.406 | 0.312 0.417 93.75 pg/ml | 0.245 | 0.243 | 0.149 0.24 46.875 pg/ml | 0.166 | 0.162 | 0.068 0.158 23.4375 pg/ml | 0.132 | 0.134 | 0.04 0.136 0 pg/ml | 0.09 | 0.094 | 0 0.098

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 74.1 | 2.3 | 3.1 Intra-assay | 2 | 20 | 218 | 9.3 | 4.3 Intra-assay | 3 | 20 | 908 | 31.1 | 3.4 Inter-assay | 1 | 20 | 74.3 | 6.7 | 9.1 Inter-assay | 2 | 20 | 220 | 14.4 | 6.6 Inter-assay | 3 | 20 | 910 | 77.1 | 8.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail