Rat Proprotein Convertase 9, Pcsk9 ELISA Kit

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 1.98 | 2.075 | 1.972 2.17 750 pg/ml | 1.229 | 1.168 | 1.065 1.107 375 pg/ml | 0.651 | 0.664 | 0.561 0.677 187.5 pg/ml | 0.401 | 0.394 | 0.291 0.386 93.75 pg/ml | 0.248 | 0.257 | 0.154 0.265 46.875 pg/ml | 0.184 | 0.177 | 0.074 0.169 23.4375 pg/ml | 0.153 | 0.144 | 0.041 0.135 0 pg/ml | 0.105 | 0.103 | 0 0.101

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 111 | 10.2 | 9.2 Intra-assay | 2 | 20 | 277 | 27.4 | 9.9 Intra-assay | 3 | 20 | 990 | 96 | 9.7 Inter-assay | 1 | 20 | 112 | 12.6 | 11.2 Inter-assay | 2 | 20 | 279 | 29.6 | 10.6 Inter-assay | 3 | 20 | 1000 | 91.6 | 9.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail