Human Receptor Tyrosine-Protein Kinase Erbb-2, ERBB2 ELISA Kit

Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.481 | 2.547 | 2.454 2.613 750 pg/ml | 1.405 | 1.342 | 1.249 1.28 375 pg/ml | 0.697 | 0.74 | 0.647 0.783 187.5 pg/ml | 0.41 | 0.406 | 0.313 0.403 93.75 pg/ml | 0.249 | 0.25 | 0.157 0.252 46.875 pg/ml | 0.171 | 0.166 | 0.073 0.161 23.4375 pg/ml | 0.119 | 0.127 | 0.034 0.135 0 pg/ml | 0.09 | 0.093 | 0 0.096

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 53.7 | 1.7 | 3.1 Intra-assay | 2 | 20 | 268 | 11.2 | 4.2 Intra-assay | 3 | 20 | 994 | 33.4 | 3.4 Inter-assay | 1 | 20 | 53.3 | 5 | 9.3 Inter-assay | 2 | 20 | 267 | 17.8 | 6.7 Inter-assay | 3 | 20 | 990 | 83.4 | 8.4

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail