Human Tumor Necrosis Factor, TNF ELISA Kit

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1) . TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & Antibody Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.316 | 2.427 | 2.346 2.538 750 pg/ml | 1.249 | 1.25 | 1.169 1.25 375 pg/ml | 0.625 | 0.652 | 0.571 0.678 187.5 pg/ml | 0.37 | 0.364 | 0.283 0.357 93.75 pg/ml | 0.206 | 0.219 | 0.138 0.231 46.875 pg/ml | 0.135 | 0.143 | 0.062 0.15 23.4375 pg/ml | 0.109 | 0.107 | 0.026 0.104 0 pg/ml | 0.077 | 0.081 | 0 0.084

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 264 | 6.8 | 2.6 Intra-assay | 2 | 20 | 575 | 22.7 | 3.9 Intra-assay | 3 | 20 | 1173 | 46 | 3.9 Inter-assay | 1 | 20 | 264 | 21.5 | 8.2 Inter-assay | 2 | 20 | 578 | 46.4 | 8 Inter-assay | 3 | 20 | 1175 | 69.8 | 5.9

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail