Human Interferon Gamma, IFNG ELISA Kit

IFN gamma, also known as IFNG, is a secreted protein that belongs to the type II interferon family. IFN gamma is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN gamma has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN gamma is critical for innate and adaptive immunity against viral and intracellular bacterial infections and tumor control. Aberrant IFN gamma expression is associated with some autoinflammatory and autoimmune diseases. The importance of IFN gamma in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1400 pg/ml | 2.55 | 2.575 | 2.465 2.601 700 pg/ml | 1.395 | 1.342 | 1.232 1.29 350 pg/ml | 0.683 | 0.732 | 0.622 0.782 175 pg/ml | 0.423 | 0.414 | 0.304 0.406 87.5 pg/ml | 0.276 | 0.268 | 0.158 0.26 43.75 pg/ml | 0.196 | 0.198 | 0.088 0.201 21.875 pg/ml | 0.151 | 0.149 | 0.039 0.148 0 pg/ml | 0.115 | 0.11 | 0 0.106

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 112 | 8.6 | 7.7 Intra-assay | 2 | 20 | 254 | 15 | 5.9 Intra-assay | 3 | 20 | 725 | 28.6 | 3.9 Inter-assay | 1 | 20 | 112 | 5.7 | 5.1 Inter-assay | 2 | 20 | 256 | 13.2 | 5.2 Inter-assay | 3 | 20 | 730 | 38.3 | 5.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail