Human Receptor Tyrosine-Protein Kinase Erbb-3, ERBB3 ELISA Kit

ErbB3, also known as Her3 (human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form. Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

Concentration | O.D. | Average | Corrected ------------------------------------------ 1200 pg/ml | 2.351 | 2.298 | 2.196 2.246 600 pg/ml | 1.331 | 1.393 | 1.291 1.455 300 pg/ml | 0.718 | 0.754 | 0.652 0.79 150 pg/ml | 0.432 | 0.442 | 0.34 0.452 75 pg/ml | 0.283 | 0.267 | 0.165 0.251 37.5 pg/ml | 0.19 | 0.182 | 0.08 0.174 18.75 pg/ml | 0.145 | 0.149 | 0.047 0.153 0 pg/ml | 0.1 | 0.102 | 0 0.105

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 109 | 5.6 | 5.1 Intra-assay | 2 | 20 | 272 | 10.8 | 4 Intra-assay | 3 | 20 | 826 | 44.4 | 5.4 Inter-assay | 1 | 20 | 110 | 10 | 9.1 Inter-assay | 2 | 20 | 270 | 10.9 | 4 Inter-assay | 3 | 20 | 825 | 50.8 | 6.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail