Human C-C Motif Chemokine 14, CCL14 ELISA Kit

CCL14 has an important biological role in other mammals by evolving under positive selection that has been lost in Ochotonidae (subgenera Pika and Lagotona) . CC chemokine ligand 14, CCL14, is a human CC chemokine that is of recent interest because of its natural ability, upon proteolytic processing of the first eight NH2-terminal residues, to bind to and signal through the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5) . Embryo implantation is a complex process involving blastocyst attachment to the endometrial epithelium and subsequent trophoblast invasion of the decidua. We have previously shown that the chemokines CX3CL1 and CCL14 are abundant in endometrial vasculature, epithelial, and decidual cells at this time, and that their receptors, CX3CR1 and CCR1, are present on invading human trophoblasts. CX3CL1 and CCL14 promote trophoblast migration.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1200 pg/ml | 2.671 | 2.683 | 2.55 2.696 600 pg/ml | 1.664 | 1.702 | 1.569 1.741 300 pg/ml | 0.924 | 0.926 | 0.793 0.929 150 pg/ml | 0.488 | 0.498 | 0.365 0.509 75 pg/ml | 0.297 | 0.312 | 0.179 0.328 37.5 pg/ml | 0.214 | 0.217 | 0.084 0.221 18.75 pg/ml | 0.172 | 0.168 | 0.035 0.164 0 pg/ml | 0.124 | 0.133 | 0 0.142

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 55.7 | 3.6 | 6.5 Intra-assay | 2 | 20 | 253 | 7.1 | 2.8 Intra-assay | 3 | 20 | 703 | 18.2 | 2.6 Inter-assay | 1 | 20 | 55.5 | 5.5 | 9.9 Inter-assay | 2 | 20 | 254 | 19.5 | 7.7 Inter-assay | 3 | 20 | 698 | 43.1 | 6.2

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail