Human Coxsackievirus And Adenovirus Receptor, CXADR ELISA Kit

CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) . Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which, D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.

Concentration | O.D. | Average | Corrected ------------------------------------------ 1200 pg/ml | 2.417 | 2.462 | 2.363 2.506 600 pg/ml | 1.301 | 1.359 | 1.26 1.416 300 pg/ml | 0.716 | 0.76 | 0.661 0.803 150 pg/ml | 0.431 | 0.433 | 0.334 0.434 75 pg/ml | 0.268 | 0.275 | 0.176 0.281 37.5 pg/ml | 0.192 | 0.189 | 0.09 0.185 18.75 pg/ml | 0.145 | 0.141 | 0.042 0.136 0 pg/ml | 0.092 | 0.099 | 0 0.105

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 57 | 3.4 | 6 Intra-assay | 2 | 20 | 219 | 12.8 | 5.8 Intra-assay | 3 | 20 | 914 | 63.4 | 6.9 Inter-assay | 1 | 20 | 57.6 | 4.5 | 7.8 Inter-assay | 2 | 20 | 219 | 22.1 | 10.1 Inter-assay | 3 | 20 | 904 | 59.1 | 6.5

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail