Human Hemopexin, HPX ELISA Kit

Concentration | O.D. | Average | Corrected ------------------------------------------ 1200 pg/ml | 2.598 | 2.591 | 2.481 2.584 600 pg/ml | 1.438 | 1.372 | 1.262 1.306 300 pg/ml | 0.802 | 0.77 | 0.66 0.738 150 pg/ml | 0.437 | 0.447 | 0.337 0.457 75 pg/ml | 0.282 | 0.272 | 0.162 0.262 37.5 pg/ml | 0.189 | 0.191 | 0.081 0.193 18.75 pg/ml | 0.147 | 0.15 | 0.04 0.153 0 pg/ml | 0.112 | 0.11 | 0 0.108

Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 70.5 | 4.8 | 6.8 Intra-assay | 2 | 20 | 160 | 5.6 | 3.5 Intra-assay | 3 | 20 | 696 | 33.1 | 4.8 Inter-assay | 1 | 20 | 70.7 | 4.3 | 6.1 Inter-assay | 2 | 20 | 161 | 8.1 | 5 Inter-assay | 3 | 20 | 704 | 39.8 | 5.7

Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail